Immunoassays and Imaging Based on Surface-Enhanced Raman Spectroscopy

Abstract

This topic covers recent developments in technologies related to biomolecular detection, including bioassay and imaging based on surface-enhanced Raman spectroscopy (SERS). Bioassay includes molecular detection technologies that monitor antigen-antibody association (immunoassay) and sequence-specific interactions between two and more complementary strands of nucleic acids (hybridization assay) as well as other molecules. Simultaneous multiple target detection and imaging (multiplexing) of samples such as cells and tissues is discussed as one of the unique features of SERS-based detection technology. Enzyme-linked immunosorbent assay (ELISA) is currently the most popular and common method for immunoassays. Bio-application of the SERS techniques focused on immunoassay and imaging is described. It covers the developments in nanostructures for substrates, configurations of measurements, imaging methods, and bio-conjugation technologies. This chapter describes experimental and instrumental methodology, such as, Raman spectroscopy, SERS, nanostructures for SERS substrates, intrinsic and extrinsic approaches, direct measurement, immunoassays using Raman-labels, SERS-based imaging, and bio-conjugation and surface-modification technologies for biological applications. SERS-based technology is regarded as one of the most powerful tools for multiplexing due to its narrow band width. Multiplexed in vivo imaging is one of the important successful achievements of SERS-based assay technology in bio-related fields. The development of table-top SERS equipment and their application to the bio-related fields would make SERS nanomaterials more popular in medical and diagnostic fields.

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Title
Immunoassays and Imaging Based on Surface-Enhanced Raman Spectroscopy
Book Title
Raman Spectroscopy for Nanomaterials Characterization
Book DOI
10.1007/978-3-642-20620-7
Chapter DOI
10.1007/978-3-642-20620-7_11
Part of
Volume
Editors
  • Challa S. S. R. Kumar (1)
  • Editor Affiliation
  • 1 Center for Advanced Microstructures and Devices, Baton Rouge, LA, USA
  • Authors
  • Dae Hong Jeong (1_11)
  • Gunsung Kim (1_11)
  • Yoon-Sik Lee (2_11)
  • Bong-Hyun Jun (2_11)
  • Author Affiliation
  • 1_11 Department of Chemistry Education, Seoul National University, Seoul, 151-742, Korea
  • 2_11 School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Korea
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