Portable SERS Sensor for Sensitive Detection of Food-Borne Pathogens

Abstract

This chapter discusses portable surface-enhanced Raman scattering (SERS) sensor for sensitive detection of food-borne pathogens. A novel assay comprising Nanoplex™ biotags, magnetic beads, and a prototype portable Raman spectrometer has been developed for rapid and simultaneous detection of enteric food-borne bacterial pathogens such as Escherichia coli O157:H7 and Salmonella species with good analytical sensitivity, accuracy, and specificity. This chapter describes genomic DNA fragmentation, probes design and synthesis, Nanoplex™ biotag synthesis and functionalization, conjugation of NeutrAvidin to SERS Nanoplex™ biotag, magnetic microsphere conjugation, DNA hybridization, and Raman instrument Nanoplex™ reader design. The coding regions of E. coli O157:H7 Stx1 gene and Salmonella enterica invA gene sequences have been chosen as the target sequences. In the multiplexed assay, the capture probe (Stx1-B1), biotinylated detection probe (Stx1-S1_biotin), and NeutrAvidin-S421 tag were applied to identify E. coli O157: H7 Stx1. The data indicate that the multiplexed assay is not only DNA sequence-specific, but also can quantitatively and simultaneously identify the different food-borne pathogens.

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Title
Portable SERS Sensor for Sensitive Detection of Food-Borne Pathogens
Book Title
Raman Spectroscopy for Nanomaterials Characterization
Book DOI
10.1007/978-3-642-20620-7
Chapter DOI
10.1007/978-3-642-20620-7_19
Part of
Volume
Editors
  • Challa S. S. R. Kumar (1)
  • Editor Affiliation
  • 1 Center for Advanced Microstructures and Devices, Baton Rouge, LA, USA
  • Authors
  • Hongxia Xu (1_19)
  • Michael Y. Sha (1_19)
  • Remy Cromer (1_19)
  • Sharron G. Penn (1_19)
  • Ed Holland (1_19)
  • Gabriela Chakarova (1_19)
  • Michael J. Natan (1_19)
  • Author Affiliation
  • 1_19 Oxonica Inc, 330 Middlefield Rd, Mountain View, CA, 94043, USA
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